Journal: Phytomedicine : international journal of phytotherapy and phytopharmacology

Article Title: Suxiao Jiuxin Pill alleviates myocardial ischemia/reperfusion-induced autophagy via miR-193a-3p/ALKBH5 pathway.

doi: 10.1016/j.phymed.2024.155359

Figure Lengend Snippet: Fig. 6. SJP alleviates autophagy through the miR-193a-3p/ALKBH5-mediated regulation of m6A methylation in vivo. (A-F) The impact of MIRI, followed by SJP, mTOR agonist MHY1485 administration, and miR-193a-3p overexpression on mTOR phosphorylation, P62, Beclin-1 expression, and LC3(II/I) ratio. (G) m6A methylation levels. (H-M) Western blot analysis of autophagy-related proteins in MIRI rat hearts influenced by SJP, METTL3 inhibitor STM2457, miR-193a-3p silencing, and the effect of ALKBH5 interference on SJP intervention. (N) m6A methylation levels. Data are presented as mean ± SD (n = 3). ***/**/*p < 0.001/ 0.01/0.05 vs. MIRI, ###p < 0.001 vs. Sham, †††/††/†p < 0.001/0.01/0.05 vs. MIRI+SJP. ns, not significant.

Article Snippet: Rats in the MHY1485 group received intraperitoneal injections of 10 mg/kg MHY1485 (#17949, CST) for one week, with an additional injection one hour before surgery on the day of the operation.

Techniques: Methylation, In Vivo, Over Expression, Phospho-proteomics, Expressing, Western Blot

Journal: Oncology reports

Article Title: Oleanolic acid induces protective autophagy in cancer cells through the JNK and mTOR pathways.

doi: 10.3892/or.2014.3239

Figure Lengend Snippet: Figure 4. OA induction of autophagy requires the suppression of the mTOR and activation of the JNK pathways. (A) A549 and PANC-1 cells were treated without or with OA (100 µg/ml) in the presence and absence of SP600125 and MHY1485. Twelve hours later, the expression of p-mTOR, mTOR, p-JNK, JNK, LC3-I, LC3-II and Beclin 1 was detected by immunoblot assays. (B and C) A549 and PANC-1 cells were transfected with a plasmid expressing LC-3-GFP. Twelve hours later, the cells containing GFP puncta were counted by immunofluorescent microscopy.

Article Snippet: Inhibitors that selectively blocked JNK (SP600125, #8177) were purchased from Cell Signaling Technology. mTOR activator MHY1485 (2 μM), Millipore, #5.00554.0001. pGFP-LC3 transfection.

Techniques: Activation Assay, Expressing, Western Blot, Transfection, Plasmid Preparation, Microscopy

Journal: Oncology reports

Article Title: Oleanolic acid induces protective autophagy in cancer cells through the JNK and mTOR pathways.

doi: 10.3892/or.2014.3239

Figure Lengend Snippet: Figure 5. The suppression of the mTOR and activation of the JNK pathways abolished the antitumor activity of OA on cancer cells. (A) A549 and PANC-1 cells were treated without or with OA (100 µg/ml) in the presence and absence of SP600125 and MHY1485. Twelve hours later, cell viability was evaluated by MTT assays. (B) The expression of cleaved caspase-3 and PARP was detected by immunoblot analysis. (C) The percentages of sub-G0/G1 population were quantified by flow cytometry. (D) Illustration of the mechanism by which OA induced autophagy and its association with apoptosis.

Article Snippet: Inhibitors that selectively blocked JNK (SP600125, #8177) were purchased from Cell Signaling Technology. mTOR activator MHY1485 (2 μM), Millipore, #5.00554.0001. pGFP-LC3 transfection.

Techniques: Activation Assay, Activity Assay, Expressing, Western Blot, Flow Cytometry